Abstract: The contents of arbutin in Rhizoma Bergeniae Scopulosae from different areas were determined using HPLC.The chromatographic separation was carried out using Inertsil ODS3 C₁₈ column（4.6 mm×150 mm，5 μm）,the mobile phase was methanol:water (8:92),the detection wavelength was set at 282 nm,the temperature was 30 ℃,and the flow rate was 1.0 mL/min.The calibration curve was linear in the range of 10.3-103.0 μg/mL and the regression equation for arbutin was Y=4468.8X-999.1 (R²=0.9999）,the average recovery was 99.58% with RSD of 1.79%（n=6）.The result showed that there are some different in the content of arbutin among different origins,the contents of arbutin were range between 18.32 mg/g to 5.34 mg /g．This method is simple,efficient and accurate,which can be used for the analysis of arbutin in Rhizoma Bergeniae Scopulosae.

Key words: Rhizoma Bergeniae Scopulosae, Bergenia scopulosa T．P．Wang, Arbutin, HPLC