关键词: 红豆杉, 紫杉醇, 肝癌细胞HEPG2, 毒力测定

Abstract: Paclitaxel is a secondary metabolite produced by plants of the genus taxuschinensis,has good curative effect for a variety of disease,which makes it become a hot spot in domestic and abroad.It is extracted from TaxusLinn leaves by the solventextraction method to explore the poisonous force of paclitaxel on liver cancer cell HEPG2,the content of paclitaxel in extractive is 1.436 μg/mg.The main composition of taxuschinensis extract is paclitaxel,which has been confirmed by HTIR.When the concentration of paclitaxel is 2 μg/mL the relative death rates of HEPG2 cells are the highest,to be 37.80%.The HEPG2 cells are handled by the taxuschinensis extract,the cells begin to turn round,enlargement,loosely arranged,cell fold,floating after 24 hours,the cells are necrosis and fragmented in 48 hours,which shows the extractive has the strong inhibition to the HEPG2 cells.The experimental results reveal that the taxuschinensis extract has the potential in the study of drug development for the treatment of liver cancer cell HEPG2.

Key words: Taxus Linn, paclitaxel, liver cancer cell HEPG2, toxicity determination