关键词: 川贝母, 分离鉴定, 内生真菌, 生物碱

Abstract: An endophytic fungus CBY4 was isolated from the bulbus of Fritillaria cirrhosa D.Don (FC) using tissue homogenate method.And this strain was identified as Fusarium tricinctum based on phylogenetic analysis and molecular biology analysis.In addition,the characteristics of fritillaria-type alkaloid (FTA) production were tested using alkaloid-chromogenic reaction,high performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and ultra performance liquid chromatography-mass spectrometry (UPLC-MS),followed by determining the yield of FTA using HPLC-ELSD method.And the results showed that F.tricinctum CBY4 could produce two kinds of FTA (peimisine and peiminine),and whose yields were 0.020 6 mg/L,0.010 4 mg/L in the culture filtrate,respectively.It is the first report that the characteristic of fritillaria-type alkaloid production of endophytic fungus from FC.And the work showed that endophytic fungal F.tricinctum CBY4 produced peimisine and peiminine,and has the potential value to produce peimisine and peiminine by fermentation techniques.

Key words: Fritillaria cirrhosa D.Don, isolation and identification, endophytic fungi, alkaloid