天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (2): 250-260.doi: 10.16333/j.1001-6880.2019.2.011

• 研究简报 • 上一篇    下一篇

不同产地川佛手中8种化学成分的分析与评价

崔广林1,2,3，李隆云1,2,3*，谭均1,2,3，张应1,2,3   

  1. 1重庆市中药研究院；2重庆市中药良种选育与评价工程技术研究中心；3中国中医科学院中药资源中心重庆分中心，重庆 400065
  • 出版日期:2019-03-07 发布日期:2019-03-07
  • 基金资助:

    重庆市现代特色效益中药材产业技术体系建设项目（2017-［5］号）；重庆市科技研发基地项目( cstc2014ptyjd10001)；国家中药材产业技术体系项目（CARS-21）；重庆市中药材产业技术体系建设项目（2018-［5］号）

Analysis and evaluation of eight active ingredients in Fructus Citris Sarcodactylis from different regions

CUI Guang-lin1,2,3,LI Long-yun1,2,3*,TAN Jun1,2,3,ZHANG Yin1,2,3   

  1. 1Chongqing Academy of Chinese Materia Medica; 2Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica; 3Chongqing Sub-center of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Science, Chongqing 400065,China
  • Online:2019-03-07 Published:2019-03-07

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摘要： 建立HPLC法同时测定不同产地川佛手中木犀草素、6,7-二甲氧基香豆素、莨菪亭、5,7-二甲氧基香豆素、7-羟基香豆素、橙皮苷、槲皮素、阿魏酸8种成分含量的方法。采用Thermo BDS HYPERSIL C18（250 mm× 4.6 mm，5 μm）色谱柱，甲醇-0.2%冰醋酸水溶液进行梯度洗脱，流速1 mL/min，柱温30 ℃，检测波长322 nm。在该色谱条件下，川佛手中8种成分分离良好，平均加样回收率为91.22%～96.52%，RSD<5.0%。不同产地的川佛手中8种成分含量存在差异，部分成分之间存在相关性，样品聚为3类。产于四川省宜宾市长宁县老翁镇的川佛手质量最优。该方法可为川佛手的资源开发利用、质量控制以及安全评价提供参考依据。

关键词: 川佛手, HPLC, 含量测定, 相关性分析, 主成分分析, 聚类分析

Abstract: To establish a HPLC method for the simultaneous determination of Luteolin,6,7-dimethoxycoumarin,Scopoletin,5,7-dimethoxy coumarin,7-hydroxy coumarin，Hesperidin,Quercetin,Ferulic acid in Fructus ctris sarcodactylis from different regions.Thermo BDS HYPERSILC18 (250 mm× 4.6 mm,5 μm) column was used，with methanol-0.2% glacial acetic acid as the mobile phases in gradient elution mode.The column temperature was 30 ℃,with flow rate 1.0 mL/min.The detection wavelength was set at 322 nm.The eight kinds of active ingredients components in Fructus ctris sarcodactylis were separated well in the chromatographic conditions.The average recoveries were between 91.22%-96.52%,RSD<5.0%.There were differences in the contents of eight components in 48 species.There was a correlation between some active ingredients.The samples of medicinal materials from 48 producing area could be divided into 3 categories.The quality of Fructus ctris sarcodactylis from Lao weng town,changning county,yibin city,sichuan province was the best.This study on active ingredients in Fructus ctris sarcodactylis can be used to provide guidance for the quality control and safety evaluation．

Key words: Fructus ctris sarcodactylis, HPLC, determination, correlation analysis, principal component analysis, cluster analysis

中图分类号: 

R284.1 R914.4

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