NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (5): 832-836.doi: 10.16333/j.1001-6880.2015.05.015

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Supercritical CO2 Extraction and Antibacterial Effects of Active Substances from Dictyophora echinovolvata

LAN Wei-qing, CAO Yi, CHEN Yan, PAN Ying-jie, SUN Xiao-hong*   

  1. College of Food Science and Technology Shanghai Ocean University,Shanghai Engineering Research Center of Aquatic Product Processing and Preservation,Shanghai 201306,China
  • Online:2015-05-30 Published:2015-06-05

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Abstract: In this paper,the antibacterial effects of active substances from Dictyophora echinovolvata prepared by supercritical CO2 extraction with different extraction conditions were studied.The orthogonal experiment L16 (43) was designed for the optimization of extraction conditions of active substances.Extraction pressure (EP),extraction temperature (ET1) and extraction time (ET2) were used as the main factors in supercritical CO2 extraction.The antibacterial effects of active substances of D.echinovolvata against Listeria monocytogenes and Vibrio parahaemolyticus were measured by minimal inhibitory concentration (MIC) and inhibitory rate.From the antibacterial effects of active substances and significance analysis for different extraction conditions,the results showed that:ET2 > EP > ET1.The optimal extraction conditions were 20 MPa,35 ℃ and 120 min.The MICs of active substances against L.monocytogenes and V.parahaemolyticus were both 15.0 mg/mL after 24 h incubation.When the concentrations of active substances were at MIC,the inhibitory rates against L.monocytogenes and V.parahaemolyticus were 85.7% and 98.2%,respectively.

Key words: supercritical CO2 extraction, Dictyophora echinovolvata;minimal inhibitory concentration;inhibitory effect

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