Extraction of Glucose Oxidase from Earthworm

doi:10.16333/j.1001-6880.2015.08.027

NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (8): 1459-1465.doi: 10.16333/j.1001-6880.2015.08.027

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Extraction of Glucose Oxidase from Earthworm

LI Jiao,YUAN Lin,RONG Yong-hai, RONG Long*

College of Biological Science and Medical Engineering,Beihang University,Beijing 100191,China

Online:2015-08-29 Published:2015-09-01

Abstract

Abstract: In this study,flash extraction was used to extract glucose oxidase (GOD) from earthworm.The extraction conditions were optimized by orthogonal test design and the optimal conditions were applied as follows:solid to liquid ratio of 1:4.5 (g/mL),flash extraction for 45 s and ultrasonic extraction for 30 min.GOD was purified by column chromatography on CM-22 and DEAE.The purified GOD had a specific activity of 2684.2 mU/mg with a yield of 60.8%.

Key words: earthworm, glucose oxidase, flash extraction, orthogonal test design, purification

CLC Number:

Q5
Q81

LI Jiao,YUAN Lin,RONG Yong-hai, RONG Long*. Extraction of Glucose Oxidase from Earthworm[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2015, 27(8): 1459-1465.

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Extraction of Glucose Oxidase from Earthworm

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