Crude samples from Aureobasidium pullulans fermentation products extracted using ethyl acetate exhibited inhibitory activity against a variety of plant pathogens,and the results showed good inhibitory activity against Cylindrocarpon destructans.To further explore the chemical constituents of A. pullulans fermentation products,ethyl acetate extract from the solid culture were separated and purified using normal-phase silica columns,reversed-phase silica columns and semi-preparative liquid chromatography,the monomer compounds were then determined mainly through analysis of NMR spectroscopic data.Four compounds were found and identified as 3,4-dimethoxy-5-methylbenzene-O-α-D-glucopyranoside (1),5′-hydroxygriseofulvin (2),7-dechlorogriseofulvin (3) and 6′-hydroxygriseofulvin (4).The above four compounds were isolated from the fermentation products of this fungus for the first time,and compound 1 was a new compound.