Abstract: The aim of this study was to establish a HPLC detection method for the simultaneous determination of 9 bioactive components (neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,loganin,rutin,cynaroside,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C) in Lonicerae japonicae Caulis.The chromatographic separation was carried out on a ZORBAX SB-C₁₈ (4.6 mm × 250 mm,5 μm) column with gradient elution of acetonitrile and 0.2% formic acid in water at a flow rate of 1 mL/min and column temperature was 30 ℃.The detection wavelength were set at 254 nm for loganin,325 nm for neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,and 350 nm for rutin,cynaroside,respectively.Excellent chromatographic separation was achieved with good linearity (R²≧0.9995) within the studied concentration ranges.The average recovery rates (RSD) of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,loganin,rutin,cynaroside,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C were 102.70% (2.54%),100.05% (329%),102.11 (1.47%),100.04% (1.32%),101.72% (0.51%),100.58% (0.51%),101.88% (1.02%), 100.55% (0.27%),101.39% (1.29%),respectively.The established method was of high sensitivity and good reproducibility,and it can be used for quality control of L.japonicae.

Key words: Lonicerae japonicae Caulis, simultaneous determination, HPLC