Abstract: It has been hard to filter or purify tremella polysaccharide extract in industrial operations because of the high viscosity of the extract.β-Mannase was used for reduce the average molecular weight and then reduce the viscosity of tremella polysaccharide extract in its extraction process.Reacting under the enzyme concentration of 0.08% for 1 h,the hydrolysis got its best result.The average molecular weight was reduced from 26480 to 16530 and the proportion of small molecules increased.With a final viscosity of 26 mPa·s,the hydrolysis did improve the filtration and purification of tremella polysaccharide extract in industrial operations.

Key words: tremella polysaccharide, Mannase, hydrolysis, viscosity, molecular weight