NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2023, Vol. 35 ›› Issue (7): 1212-1221.doi: 10.16333/j.1001-6880.2023.7.013

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Effect of decocting time on chemical composition and anti-fatigue effect of Triphala decoction

DENG Xuan1,QIU Min1,HUANG Hao-zhou2,FAN San-hu3,ZHANG Ding-kun1,LUO Chuan-hong1,HAN Li1*,LIN Jun-zhi4*   

  1. 1State Key Laboratory of Southwestern Chinese Medicine Resources,Pharmacy School,Chengdu University of Traditional Chinese Medicine;2Innovative Institute of Chinese Medicine and Pharmacy of Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;3Sanajon Pharmaceutical Group,Chengdu 610000,China;4TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610072,China
  • Online:2023-07-31 Published:2023-07-31


To explore the effect of decocting time on the component transformation and anti-fatigue effect of Triphala decoction.HPLC method was used to track the changes of ingredients in Triphala decoction during decoction.Twelve differential compounds were screened through partial least squares discriminant analysis (PLS-DA) and seven of these were identified as gallic acid,chebulic acid,chebulinic acid,chebulagic acid,ethyl gallate,ellagic acid and corilagin.During the decoction,the contents of chebulinic acid,chebulagic acid,ethyl gallate and ellagic acid all decreased while the gallic acid and chebulic acid increased.Corilagin′s content increased first,then reduced.The anti-fatigue effect of Triphala decoction with different extraction times was then evaluated.The longer the decoction time,the more potent the anti-fatigue effect was,according to the results of swimming time and 11 fatigue-related markers in mice.Gallic acid and chebulic acid,among the differential compounds,have the greatest impact on the anti-fatigue effect.It demonstrates how decocting can encourage the hydrolysis of the macromolecular tannins in Triphala decoction into gallic acid and chebulic acid,which can improve the anti-fatigue effect of Triphala decoction.

Key words: Triphala decoction, decocting time, polyphenols, hydrolysis, anti-fatigue effect

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