Abstract: To establish a capillary electrophoresis method for the determination of 5α-reductase activity, rat liver testosterone 5α-reductase catalytic activity was determined. Large volume injection and sweeping micellar push method to 20 mmol/L borax 60 mmol/LSDS pH 9.20 running buffer, voltage 20 kV, detection wavelength 242 nm, the extracted rat liver 5α-reductase activity was monitored. Through the optimization of electrophoresis conditions within 10 min achieved 5α-reductase activity was measured. As a conclusion, a capillary electrophoresis method was successfully developed for the determination of 5α-reductase activity.

Key words: 5α-reductase, capillary electrophoresis, micellar sweeping, Adiantum capillus-junonis