NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (Suppl): 122-127. doi: 10.16333/j.1001-6880.2015.S.030

• Article • Previous Articles     Next Articles

Reduce the Viscosity of Tremella Polysaccharide Extract with β-Mannase

FAN Lin-na1, JIA Yan1, SONG Li-ya1, WANG Ling2, HE Cong-fen1   

  1. 1 Beijing Key Laboratory of plant resources research and development;School of Science,Beijing Technology and Business University;100048,Beijing,China;2 Beijing Huaxiazhongfang Biotechnology Co.Ltd.100036,Beijing,China
  • Online:2015-12-01 Published:2016-01-12

Abstract: It has been hard to filter or purify tremella polysaccharide extract in industrial operations because of the high viscosity of the extract.β-Mannase was used for reduce the average molecular weight and then reduce the viscosity of tremella polysaccharide extract in its extraction process.Reacting under the enzyme concentration of 0.08% for 1 h,the hydrolysis got its best result.The average molecular weight was reduced from 26480 to 16530 and the proportion of small molecules increased.With a final viscosity of 26 mPa·s,the hydrolysis did improve the filtration and purification of tremella polysaccharide extract in industrial operations.

Key words: tremella polysaccharide, Mannase, hydrolysis, viscosity, molecular weight

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