Abstract: Ant protein were used as the material to extract polypeptide by Alkaline protease.Response surface methodology(RSM) was employed to maximize Content of peptides (R₁) and DPPH Scavenging rate (R₂) based on a central composite design involving 3 factors (pH,Extraction time and Enzyme substrate ratio)at 3 levels,Hydrolysis condition optimization of ant protein enzyme analysis by means of 3 factors and 3 levels of response surface methodology of Box-Behnken. Results showed the optimal process parameters is the pH value 9.3,extraction time 5.2 h,Enzyme substrate ratio concentration 0.95%.Under such conditions,the observed values of R₁ 2.000mg/mL and R₂ 43.24%,respectively in close agreement with predicted values.This reveals the applicability of RSM to the optimization of Alkaline Protease enzymolysis process of Ant protein.

Key words: ant, response surface methodology, enzymolysis, content of peptides, DPPH scavenging rate