Abstract: The inhibitory effects of chestnut flower flavonoids extracted by semi-bionic,ethanol reflux or water methods on the proliferation of cervical cancer Hela cells were studied by MTT method with human renal epithelial 293T cell as control.The results showed that chestnut flower flavonoids extracted by above three methods exhibited the powerful cytotoxic activity on Hela cells in the range of experimental concentration and the inhibitory effect was in dosedependent manner.The half maximal inhibitory concentration (IC^₅₀) values of semibionic,ethanol reflux and waterflavonoids were 7.30,9.94,22.48 μg/mL for Hela cells,and 54.71,54.17,153.44 μg/mL for 293T cells,respectively.Especially,when the concentrations of chestnut flower flavonoids were lower than 30 μg/mL,the inhibitions of chestnut flower flavonoids were significant on the proliferation of Hela cells (P<0.05),but not on 293T cells (P>0.05).Therefore,flavonoids of chestnut flower had good inhibitory effect on the proliferation of Hela cells,and among the flavonoids extracted by three methods,the semibionicflavonoids showed the most powerful inhibitory effect on Hela cells.

Key words: chestnut flower, flavonoids, extraction method, cell proliferation