Abstract: The aim of this study was to optimize the inclusion technology of volatile oil with β-CD and to use appropriate methods for identification.Three methods including saturated water solution,grinding and ultrasonic were investigated to prepare inclusion compound.And the method was established based on the inclusion rate of volatile oils,yield of inclusion compound and oil content.After that,the formulation and processing parameters were optimized by L₉(3⁴) orthogonal design.The formations of inclusion complex were validated by UV,TLC,DSC and microscopic imaging.The optimal preparation conditions for inclusion were established as follows:the method of saturated solution stirring was the best and volatile oil: β-CD was 1:8,the inclusion temperature was 40 ℃ and the time was for 3 hours and the inclusion temperature was at 40 ℃.Analysis results showed that the inclusion technique was successful.The process was simple and it can improve the stability and solubility of volatile oil effectively.Furthermore the method can be used for industrial production of Tingli Shengmai decoction.

Key words: Rhizoma Atractylodis macrocephalae volatile oil, Ramulus cinnamomi volatile oil, orthogonal test, &beta, -cyclodextrin inclusion, identification