Abstract: To discuss the mechanism of modified Rhizoma Alismatis Decoction on prevention and treatment of hyperlipemia,through investigating hepatic expressions of AQP3 in rats with hyperlipemia.60 Male Sprague-Dawley rats were randomly divided into 6 groups:Control group,Model control group,Simvastatin control group,High-dose of modified Rhizoma Alismatis Decoction group,Middle-dose of modified Rhizoma Alismatis Decoction group,Low-dose of modified Rhizoma Alismatis Decoction group.Rats in control group were fed with ordinary forage,and the others were fed with high-fat forage.At the same time,each treatment group was administrated with appropriate drug.Then TC,TG,HDL-C and LDL-C of serum were detected,the genetic expression of AQP3 in colon tissue were detected with RT-PCR and the protein expression of AQP3 in colon tissue were detected with immunohistochemical means and Western Blot after 5 weeks.In the model control group,the content of TG,TC,LDL-C in serum and the expression of AQP3 in colon tissue were significantly elevated,while the content of HDL-C was significantly reduced.After treatment,the contents of TG,TC,LDL-C and the expression of AQP3 in every treatment groups were reduced,while the contents of HDL-C were elevated.Modified Rhizoma Alismatis decoction had a satisfying effect on regulating the blood lipid.Increasing the genetic and protein expression of AQP3 in colon tissue,regulating the water fluid metabolism,preventing the formation of sputum and removing the pathogenic factors could be viewed as mechanisms of modified Rhizoma Alismatis Decoction concerning prevention and treatment of hyperlipemia.

Key words: modified Rhizoma Alismatis decoction, hyperlipidemia, AQP3, experimental research