Abstract: In this paper,a new separation technology,ionic liquidbased three phase partitioning (ILTPP) systems was used.Bovine serum albumin was used as the model protein in these systems and ionic liquid replaced traditional tbutanol.Ionic liquid,salt and protein formed new phases.In this experiment,the mass of ionic liquid,the mass of ammonium sulfate,pH,and temperature were treated as experimental factors.Finally,the recovery efficiency can reach 98%.The result shows that ionic liquid can replace tbutanol.ILTPP for recovery protein is a promising method.

Key words: ionic liquidbased three phase partitioning, protein, recovery, response surface