NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2017, Vol. 29 ›› Issue (增刊1): 171-175. doi: 10.16333/j.1001-6880.2017.S.034

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Recovery of Bovine Serum Albumin Using Ionic liquid-based Three Phase Partitioning Systems

ZHAO Cong-cong1,LI Fen-fang1*,WANG Run-ping1,TAN Zhi-jian2   

  1. 1 School of Chemistry and Chemical Engineering,Central South university,Changsha 410083,China; 2Institute of Base Fiber Crops,Chinese Academy of Agricultural Science,Changsha 410205,China;
  • Online:2017-08-02 Published:2017-08-02

Abstract: In this paper,a new separation technology,ionic liquidbased three phase partitioning (ILTPP) systems was used.Bovine serum albumin was used as the model protein in these systems and ionic liquid replaced traditional tbutanol.Ionic liquid,salt and protein formed new phases.In this experiment,the mass of ionic liquid,the mass of ammonium sulfate,pH,and temperature were treated as experimental factors.Finally,the recovery efficiency can reach 98%.The result shows that ionic liquid can replace tbutanol.ILTPP for recovery protein is a promising method.

Key words: ionic liquidbased three phase partitioning, protein, recovery, response surface