NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2018, Vol. 30 ›› Issue (2): 286-293.doi: 10.16333/j.1001-6880.2018.2.019

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 Characteristics and antioxidant ability of squid skin collagen

SONG Zheng-gui, LEI Jing, XUE Zhang-zhi, ZHANG Hong-chao, LI He-sheng*, WANG Hong-fei, FU Jing-jing   

  1. Ocean College of Ningbo University,NingBo 315211,China
  • Online:2018-02-28 Published:2018-02-28

Abstract: In order to improve the comprehensive utilization rate of squid,squid-skin were used as the main raw materials to extract collagen,then the characteristics of collagen extracted from tartaric acid were comprehensively evaluated.The results showed that the collagen had a high solubility,There were a minimum solubility of collagen at the pH of 5,decreased by 46%.When the concentration of NaCl was greater than 0 mol/L,the solubility of collagen decreased as the salt concentration increased.And while the concentration of NaCl increased to 1.2 mol/L,the solubility of collagen decreased by 80%.The collagen concentration,pH,ionic strength and storage time have an effect on viscosity.The viscosity gradually increased with the increased of collagen concentration.The viscosity peaked at pH3.With the increase of NaCl concentration,the viscosity decreases gradually.With the prolonging of the storage time,the viscosity presented a trend of decrease after the first increase,and the viscosity of 24-48 h was the largest.The antioxidant capacity increased with the increase of collagen concentration.Increased collagen concentration,the eliminating ratio of increased gradually, which is 5mg/mL when the maximum file size is reached, 49.66%.Within the range of 2 mg/mL-10 mg/mL,little change in reducing capacity,After more than 10 mg/mL,itsignificantly increased, but still relatively low.With the increase of collagen concentration,the eliminating ratio of hydroxyl radical increased,At 10 mg/mL,the removal rate reached a maximum of 95.66%.This paper supplied the theoretical basis for making full use of the squid-skin collagen.

Key words: protein of collagen from cuttlefish skin, collagen;solubility, viscosity, antioxidant capacity

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