NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2018, Vol. 30 ›› Issue (3): 385-389.doi: 10.16333/j.1001-6880.2018.3.007

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Aβ1-42  Oligomer Induces the Expression of TNF-α through PI3K-AKT Signaling Pathway in Neurogliocyte

ZHANG Hai-bo1*, SUN Yang2   

  1. 1The Second Department of Neurosurgery,the Affiliated Central Hospital of Shenyang Medical College,Shen Yang 110024,China; 2Shenyang First Aid Center,Shen Yang 110000,China  
  • Online:2018-04-08 Published:2018-04-08

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Abstract: In this study,Western blot and Real-Time PCR methods were used to investigate the effects of Aβ1-42 oligomers on TNF-alpha expression in human glioblastoma A172 and murine glioblastoma D1A cells by AKT signaling pathway,at the same time,the specific regulation mechanism of Aβ1-42 oligomer induced TNF-alpha expression was detected by immunofluorescence assay.The results showed that Aβ1-42 oligomers can upregulate the expression of inflammatory factor TNF-alpha after treating A172 and D1A cells with Aβ1-42oligomers,and this induction is achieved via the PI3K-AKT signaling pathway.Based on this experimental study,we demonstrate that Aβ1-42oligomers participate in the inflammatory reaction in the AD brains,and suggest that anti-inflammation and anti Aβ have important theoretical significance in the clinical treatment of Alzheimer's disease.

Key words: Aβ1-42, TNF-α, Glial cells, AKT signaling pathway

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