NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2018, Vol. 30 ›› Issue (增刊1): 124-128.doi: 10.16333/j.1001-6880.2018.S.022

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Determination of GABA Content in Compound Aminobutyric Acid and Vitamin E Capsule by HPLC-CAD

CHEN Hong-ying 1, LIN Geng-xue 2, WANG Jian-song1, YUAN Xiao2,3*   

  1. 1Guangzhou Bai Yun Shan pharmaceutical group co.,LTD.,Bai Yun Shan Pharmaceutical Factory,Guangzhou，510515,China; 2Guangzhou PI & PI Biotech Inc.Ltd.,Guangzhou,510540,China; 3Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan,430074,China  
  • Online:2018-07-31 Published:2018-07-31

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Abstract: This experiment was done by using HPLC combined with CAD.Hypersil GOLD C18 was used as the chromatographic column.The mixture solution of Nonafluoropentanoic acid (0.05% in water) and methanol with a proportion of 97∶3 was used as the mobile phase for the isocratic elution.Determination of GABA content in compound aminobutyric acid and vitamin E capsule was done by comparison of the described curve and equation of the standard GABA compound.The Linear equation in this experiment was described as y=-0.00004x2+0.0602x+1.6924,r=0.9997,with the linear range of 52~415 μg*mL-1.The mean recovery of samples was 101.6% and RSD% was 1.9% (n=9).This method is simple and convenient,which could give a rapid determination of GABA content in aminobutyric acid vitamin E capsule.

Key words: aminobutyric acid vitamin E capsule, GABA, CAD, HPLC  

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