Abstract: The active ingredients of animal traditional Chinese medicines are mainly proteins and polypeptides,so active proteins and polypeptides have important medical and health value.In this paper,the basic principles and main application scopes of precipitation method,chromatography,membrane separation method and electrophoresis method are analyzed.The application of these methods in the separation and purification of animal-derived active proteins and polypeptides is reviewed.This provides a reference for the separation,purification and further research of animal-derived proteins and polypeptides,in order to develop a new technology for the separation and purification of proteins and polypeptides with high efficiency,economy and environmental protection.

Key words: proteins, polypeptides, separation, purification, research progress