Abstract: In order to explore the overall difference of the functional components of the fruit stem and leaf of Hippophae rhamnoides,ultra-high performance liquid chromatography coupled to quadrupole Orbitrap high resolution mass spectrometry (UPLC-Q-Exactive) combined with database of Orbitrap traditional Chinese medicine library (OTCML) used in this study.The chromatographic column is Hypersil GOLD aQ column (2.1 mm×100 mm,1.9 μm).Meanwhile 0.1% formic acid acetonitrile (A)-0.1% formic acid water (B) gradient elution as the mobile phase,flowing at rate 0.3 mL/min.High resolution mass spectrometry with electrospray ionization (ESI) set positive and negative scanning mode at the same time.Based on the retention time,molecular ion,secondary fragment ion spectrum information and literature data of more than 1 200 kinds of traditional Chinese medicine compounds contained in OTCML database,then the structure was identified.As results,102 active components were identified,including 18 glycosides,21 flavonoids,18 terpenoids,13 phenylpropanoids,6 alkaloids,14 volatile components and 12 polyphenols.Seventeen of these compounds were first found in Hippophae rhamnoides.There were obvious differences in the distribution of different parts.This method provides basic data for the development and utilization of Hippophae rhamnoides resources in the future,and also lays a foundation for the quality control of Hippophae rhamnoides related products and the basic research on the substance of medicinal effects.

Key words: UPLC-Q-Exactive, Hippophae rhamnoides, functional components, OTCML