NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2019, Vol. 31 ›› Issue (9): 1559-1566.doi: 10.16333/j.1001-6880.2019.9.011

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Simultaneous determination of six flavonoids in Psidium guajava Linn.by QAMS

FANG Hao,XIONG Su-qin,YAN Na-na, CHEN Lin,CHEN Hong-ping,LIU You-ping*   

  1. Pharmacy College,Chengdu University of Traditional Chinese Medicine,Breeding Base of State Key Laboratory of Resources System Research and Development Utilization of Chinese Herbal Medicines Co-constuctioned by Ministry of Science and Technology and Sichuan Province,Chengdu 611137,China

  • Online:2019-10-09 Published:2019-10-23

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Abstract: In this study,a new method of quantitative analysis of multi-components by single marker (QAMS) method was developed for simultaneously determining six flavonoids(hyperoside,isoquercitroside,reynoutrin,guaijaverin,avicularin,taxifolin) in Psidium guajava Linn..Using hyperoside as the internal reference marker,the relative correction factor (RCF) of hyperoside for other five chemical components was established by HPLC,and the content of other five components were calculated by RCFs.Meanwhile,the contents were also determined by the external standard method.The results indicated that no significant differences between QAMS and external standard method.Therefore,QAMS is suitable for quantitative analysis of six flavonoids in Psidium guajava Linn..

Key words: quantitative analysis of multi-components by single marker (QAMS), Psidium guajava Linn., relative correction

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