NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2020, Vol. 32 ›› Issue (8): 1343-1347.doi: 10.16333/j.1001-6880.2020.8.009

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Chemical constituents from berries of Brassica rapa L. ssp. pekinensis

WANG Han,SI Han-rui,JIAO Yu-feng,LIU Jin-ping,LI Ping-ya*#br#


  1. College of Pharmacy,Jilin University,Natural Medicine Research Center,Jilin University,Changchun 130021,China

  • Online:2020-08-28 Published:2020-09-02


To investigate the chemical constituents from the Brassica rapa L. ssp. pekinensis (Cruciferae).The constituents from the 95% ethanol extract of Brassica rapa L. ssp. pekinensis were separated and purified by silica gel chromatography,Sephadex LH-20,semi-preparative high performance liquid phase,crystallization,recrystallization technologies.The structures of isolated compounds were elucidated by physicochemical properties and spectral data analysis.Fifteen compounds were isolated and purified.They were identified as β-sitosterol(1),5-hydroxymethylfurfural (2),n-butyl-β-D-fructopyranoside (3),(6S,9S)-roseoside(4),uridine(5),succinate(6),methyl succinate(7),p-hydroxycinnamic acid(8),ethyl-β-D-glucopyranoside (9),butyl pyroglutamate(10),cyclo (Pro-Leu)(11),cyclo(Tyr-Leu)(12),1-chlorohexadecane(13),oleamide(14),stigmastane-3,6-dionez(15).Compounds 3 and 6-15 are obtained from the plants of Brassicas.for the first time,and compound 1,3-15 is isolated from Brassica rapa L. ssp. pekinensis for the first time.The effect of the compound 3 on inhibiting the growth of laryngeal carcinoma Hep-2 cells in vitro was examined by MTT.

Key words: Brassica rapa , L. ssp. , pekinensis, extraction and separation, structural identification, activity screening

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