NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2020, Vol. 32 ›› Issue (8): 1389-1395.doi: 10.16333/j.1001-6880.2020.8.015

Previous Articles     Next Articles

Enzyme-assisted extraction of polysaccharides of Ta-pieh Mountains Bletilla striata and its activities study

ZHU Fu-cheng,LUO Shu-lan,ZHENG Xuan,WANG Fang,HE Xiao-mei,DENG Hui,HAN Bang-xing*#br#


  1. College of Biology and Pharmaceutical Engineering,Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources,West Anhui University,Luan 237012,China

  • Online:2020-08-28 Published:2020-09-02


Bletilla striata polysaccharides (BSP) have attracted extensive research interest due to their potential application of medicinal value and skin care. Herein,enzymatic assisted technique was applied to extract BSP from B. striata of Ta-pieh Mountains for the first time. Firstly,response surface methodology was applied to improve extraction efficiency. Under the optimal conditions,the maximum extraction yield was 64.8%. Scanning electron microscope and spatial conformation analysis shown that enzymatic extraction did not destroy the natural structure of polysaccharides. Then,antioxidant activity of extracted BSP was analyzed,and the results clearance rates of hydroxyl radical,superoxide anion and DPPH were 87.6%,71.5% and 83.9%,respectively,at the finite concentration of BSP,which presented a good antioxidant activity. The extraction yield of BSP was highest report to data. The efficient extraction of the BSP in this study lays the foundation for its industrial application.

Key words: Bletilla striata of Ta-pieh Mountains, Bletilla striata , polysaccharides, enzyme-assisted extraction, response surface methodology, antioxidant activity

CLC Number: