NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2021, Vol. 33 ›› Issue (3): 453-461.doi: 10.16333/j.1001-6880.2021.3.013

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Physicochemical properties and antioxidant activities of melanin from Chinese soft-shelled turtle (Pelodiscus sinensis)

XIANG Jin-min,ZHAO Qiong-yu,YUAN Hang,PENG Zhen-hu,SONG Wei*   

  1. College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,China
  • Online:2021-03-28 Published:2021-04-01


This study aimed to develop a method for extracting melanin in muscle of Chinese soft-shelled turtle by Skim-enzymatic hydrolysis and acid hydrolysis,and to study its structural characteristics,physicochemical property,as well as antioxidant activity.The melanin was examined by ultraviolet-visible spectroscopy,fourier transform infrared spectroscopy and elemental analyzer,before being evaluated by color detection,solubility test,stability test,hydroxyl radical assay,DPPH radical-scavenging assay and superoxide-radical scavenging assay.The result showed that the melanin powder was dark mixed with a little red and yellow (L* = 41.03,a* = 2.26,b* = 3.78).The solubility properties were similar to those of typical melanin.The melanin possessed a stronger thermal stability and light stability within 1 hour,as well as exhibited a strong antioxidant activity.At concentration of 2.0 mg/mL,the total antioxidant activity,hydroxyl radical scavenging activity,DPPH radical scavenging activity,and superoxide-radical scavenging activity of melanin were reduced more than 40%.

Key words: Pelodiscus sinensis, melanin, structural characteristics, physicochemical property, antioxidant activity

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