To explore the mechanism of Aconiti Lateralis Radix Preparata-Zingiberis Rhizome (AZ) against myocardial ischemia-reperfusion injury (MI/RI) by network pharmacology.To search the active components and corresponding targets of AZ through the TCMSP databases.MI/RI targets were obtained from Disgenet database.Constructed protein interaction network map by String online database.At the same time GO and KEGG enrichment analysis of key targets were carried out by using R language.The results of network pharmacologic prediction were verified by cell experiments.A total of 16 AZ active compounds and 171 targets were obtained.GO analysis showed that AZ may play a role in the treatment of MI/RI through apoptosis,inflammation and vasodilation.KEGG analysis showed that AZ may play a therapeutic role through PI3K-Akt signaling pathway,TNF signaling pathway and HIF signaling pathway.Experimental verification showed that AZ could improve the survival rate of vascular endothelial cells,reduce the apoptosis rate and oxidative damage of rats with hypoxia reoxygenation injury.Moreover,the expression of HIF-α,VEGF and eNOS protein was increased.AZ treatment of MI/RI can reduce the oxidative damage and apoptosis rate of vascular endothelial cells by activating the HIF/VEGF/eNOS signaling pathway.

"> network pharmacology, Aconiti Lateralis Radix Preparata-Zingiberis Rhizoma, vasodilation, myocardial ischemia-reperfusion injury