Abstract: To study the chemical constituents of Murraya paniculata (L.) Jack, two new compounds, named as murrayanin (1) and 8-demethylnobiletin (2), along with twenty-four known compounds (3-26) were isolated from the 70% EtOH extract of M. paniculata by various chromatographic techniques such as silica gel, ODS, Sephadex-LH 20, Pre-HPLC, their structures was elucidated by spectral data analysis. The known compounds were identified as ficusal (3),lariciresinol-4′-monomethy ether (4),(±)-5′-methoxy-4′-O-methyllariciresinol (5),diospyrosin (6),(-)-9′-O-E-feruloyl-lyoniresinol (7),7-O-methylphellodenol-B (8),osthenon (9),3,4′-dihydroxy-3′,5′-dimethoxyphenylacetone (10),4′-hydroxy-5,7-dimethoxyflavanone (11),cystosiphonin (12),4′-hydroxy-5,7,3′-trimethoxyflavanone (13),5,7,3′,4′,5′-pentamethoxyflavanone (14),2′,4-dihydroxy-3′,4′,6′-trimethoxychalcone (15),2′,3-dihydroxy-4,4′,6′-trimethoxychalcone (16),evofolin B (17),2′-hydroxy-3,4,5,4′,6′-pentamoxychalcone (18),2′-hydroxy-3,4,4′,6′-tetramethoxychalone (19),5,8-dihydroxy-6,7,3′,4′-tetramethoxyflavone (20),3′-hydroxy-5,6,7,8,4′,5′-hexamethoxyflavone (21),5,3′,5′-trihydroxy-7,4′-dimethoxyflavone (22),5,7,3′-trihydroxy-8,4′-dimethoxyflavone(23),8-hydroxy-5,6,7,3′,4′-pentamoxyflavone (23),3′-hydroxy-5,6,7,4′-tetramethoxyflavone (24),5,7,3′,4′,5′-pentamethoxyflavone (25) and 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone (26)，respectively.Compounds 3-8,10-13,15,16,20-24 were obtained from the genus Murraya for the first time,and compound 17 were isolated from M. paniculata for the first time.

Key words: Murraya paniculata (L.) Jack., chemical constituent, lignan, flavone, coumarin