NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2023, Vol. 35 ›› Issue (6): 915-924.doi: 10.16333/j.1001-6880.2023.6.001

    Next Articles

Optimization of the extraction process of total phenolic acid from Trollii Flos by response surface methodlogy and study on its whitening activity

DU Ruo-jun1,SHI Zhao-hua1,ZHAN Zhi-lai2,HU Jun-jie1*,ZHENG Guo-hua1*   

  1. 1School of Pharmacy,Hubei University of Traditional Chinese Medicine,Wuhan 430065,China;2Traditional Chinese Medicine Resource Center,China Academy of Chinese Medical Sciences,Beijing 100700,China
  • Online:2023-06-28 Published:2023-06-28

Abstract:

Box-Behnken design experiments in response surface analysis were used to optimize the process conditions for the ethanol reflux extraction of total phenolic acid from Trollii Flos,and the scavenging ability of total phenolic acid from Trollii Flos on DPPH radicals and hydroxyl radicals and its inhibitory effect on tyrosinase were also studied.The results showed that the optimum extraction process of total phenolic acid from Trollii Flos was:ethanol concentration 60%,reflux time 90 min,reflux temperature 60 ℃,liquid to solid ratio 40∶1 (mL/g).The yield of total phenolic acid from Trollii Flos under these conditions was 92.245 mg/g.In addition,it had certain scavenging ability to DPPH radicals and hydroxyl radicals,and could significantly inhibit tyrosinase activity.The extract method is stable and reliable,and the total phenolic acid from Trollii Flos has certain in vitro antioxidant activity and tyrosinase inhibitory effect,which lays the foundation for further scientific and rational development and utilization of Trollii Flos.

Key words: Trollii Flos, total phenolic acid, response surface, antioxidation, tyrosinase, whitening

CLC Number: