To establish the UPLC characteristic chromatogram of Verbenae Herba and quantitative analysis of multi-components by single marker(QAMS) for the hastatoside,cornin and verbascoside,a total of five characteristic common peaks were identified and three components were identified for the analysis of 18 batches of Verbenae Herba from different habitats.The similarity of 18 batches of samples was greater than 0.830.The errors were all less than 3% when compared with the results measured by the external standard method.hastatoside,cornin and verbascoside in 18 batches of standard decoction were 17.36-51.34 mg/g,7.76-72.85 mg/g,1.15-15.44 mg/g.The transfer rates of 18 batches of Verbenae Herba to standard decoction hastatoside,cornin and verbascoside were 35.0%-113.4%,52.1%-109.2%,7.9%-36.3%.The simple and feasible established characteristic chromatogram combined with QAMS can provide reference and basis for the overall quality control of Verbenae Herba.