Abstract:

This study aims to optimize the enzyme-assisted aqueous two-phase extraction process of chicory root polysaccharides (CRP) and evaluate its lipid-lowering activity.The research employed the Box-Behnken design and response surface methodology to optimize the extraction process,examining the effects of the amounts of cellulase and pectinase added,the mass fraction of (NH₄)₂SO₄,and the mass fraction of polyethylene glycol (PEG) on the yield of CRP.The results indicated that the amounts of cellulase,pectinase,(NH₄)₂SO₄ mass fraction,and PEG mass fraction all significantly affected the yield of polysaccharides.The optimized extraction conditions were cellulase amount of 2.00%,pectinase amount of 2.00%,(NH₄)₂SO₄ mass fraction of 18.00%,and PEG mass fraction of 15.00%,under which the yield of polysaccharides can reach 32.78%.Additionally,this study found that CRP can effectively reduce lipid deposition in fatty HepG2 cells,decrease total cholesterol and triglyceride levels,indicated their good lipid-lowering activity.

Key words:

"> chicory root polysaccharides, enzymatic assisted aqueous two-phase extraction, response surface methodology, lipid-lowering activity