Abstract: The enrichment process of polymethoxylated flavones from dried tangerine or orange peel using macroporous resin were optimized.Based on the contents of nobiletin and tangeretin determined by HPLC,the adsorption capacity and elution ratio of different resins were studied and compared.D101 macroporous resin was most suitable for separation and purification of polymethoxylated flavones from orange peel.The concentration and dosage of eluent,sample concentration were investigated and the optimal conditions were obtained as follows:the eluent was 80% ethanol,dosage of eluent was 6 BV,sample concentration was 500 mg/mL.After processing,the purities of nobiletin and tangeretin were improved 3.7 times and 3.2 times,respectively.The results proved that macroporous resin can be used for separation and purification of polymethoxylated flavones from orange peel.

Key words: orange peel, macroporous resin, polymethoxylated flavones, nobiletin, tangeretin