NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (10): 1618-1623.

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Identification and Purification of Paeoniflorins in Seed Cakes of Paeonia sufruticosa by Macroporous Adsorption Resin

QIN Ai-xia1,JI Shu-jing1,MAO Wen-yue2,GAO Zhe1,KANG Xiao-hu1,JIA Ya-nan1,CHEN Can1,CUI Tong1*   

  1. 1 College of Food Science and Technology,Agricultural University of Hebei,Baoding 071000, China; 2 Heze Yao and Shun Peony Biotechnology Co.,LTD,Heze 274000 , China
  • Online:2014-10-30 Published:2014-10-31

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Abstract: Four main paeoniflorins,namely 6′-O-β-D-glucopyranosylalbiflorin,albiflorin, β-gentiobiosylpaeoniflorin and paeoniflorin were isolated from the alcohol extract of seed cakes of Paeonia sufruticosa Andr.and identified by LC-MS and1H-NMR.The purification procedure using macroporous adsorption resins was optimized.The results showed that HPD200A resin was the most suitable resin among the 11 tested resins.The optimal procedure for the purification was as follows:the concentration of sample solution was 8.0 mg (paeoniflorin)/mL,the loading capacity was 4.5 bed volume (BV) and the flow rate was 1/16 BV/min;then eluted with 50% ethanol for 4 BV with elution flow rate of 1/16 BV/min.Under these optimal conditions,the content of paeoniflorin,albiflorin,6′-O-β-D-glucopyranosylalbiflorin and β-gentiobiosylpaeoniflorin was reached to 32.3%,16.5%,8.02% and 6.63%,respectively.

Key words: Paeonia sufruticosa, seed cake, paeoniflorin, albiflorin, macroporous adsorption resin

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