Abstract: Endophytic Pestalotiopsis versicolor was fermented in plenty liquid culture,A variety of chromatographic purification methods were applied,and eight compounds were isolated from its metabolites.Their structure was identified according to their physicochemical properties and spectral data,they are as follows:ergosterol(1)，3β,5α,9α-Trihydroxy-7,22-ergosterol diene-6-ketone(2),ergossta-8(9),22-diene-3,5,6,7-tetraol(3β,5α,7α,22E)(3),butyl 2-pyrrolidone-5-carboxylate(4),5methylpyrimidine-2,4-dione(5),pyrimidine-2,4-dione(6)，1,2-benzenedicarboxylic acid,dibutyl ester(7),p-hydroxybenzaldehyde(8).

Key words: endophytic fungus, Pestalotiopsis versicolor, chemical constituents