Abstract: Ten compounds were separated and purified from the ethyl acetate fraction of the alcohol extract of Rhizoma Phragmitis by silica gel and Sephadex LH-20 column chromatography.Their structures were identified as benzoic acid (1),D-allitol (2),acetyloleanolic acid (3),borreriagenin (4),3′,7-dihydroxy-4′-methoxyisoflavon (5), oleanolic acid (6),(-)-chicanine (7),3β,5α,9α-trihydroxyergosta-7,22-dien-6-one (8),betulinic acid (9) and umbelliferone (10).All the compounds were isolated from this species for the first time.

Key words: Rhizoma Phragmitis, extraction, isolation, structural identification