Abstract: This experiment explored water bath extraction and supersonic to extract the content of total flavonoids from bamboo leaves.Via single factor experiment and orthogonal test,two methods optimal extract parameters were acquired.The results showed the optimal parameters for the extraction of total flavones from bamboo leaves by water bath method:Ethanol concentration 90%,liquidsolid ratio of 25:1(mL/g),extraction time 0.5 h,extraction temperature(water bath)70 ℃,for 3 times,the extracting rate was 1.79%.The optimal parameters for the extraction of total flavones from bamboo leaves by supersonic:Ethanol concentration 90%,liquidsolid ratio of 35:1(mL/g),extraction time 75 min,extraction power 360 W,for twice,the extracting rate was 2.75%.The method of supersonic was better than water bath.

Key words: Bamboo leaves, Total flavonoids, Extraction, Water bath, Supersonic, Optimization