Abstract: To study the chemical constituents in methanol fraction of Galium aparine L.,the 75% alcohol extract of G.aparine were subjected to silica gel,ODS and Sephadex LH-20 column chromatography.The isolates were identified with the physicochemical properties and spectra data.Eight compounds from G.aparine were identified as 6-O=acetylscandoside(1),6α=hydroxy geniposide(2),geniposidic acid(3),6β-hydroxy geniposide(4),daphylloside(5),6α-methoxy geniposidic acid(6), deacetylasperulosidic acid(7), lucidin-3-O-glucoside(8).Compounds 1-6 were isolated from G.aparine for the first time,and compounds 4 and 6 were firstly isolated from the genus Galium.

Key words: Galium aparine, iridoid glycoside, 6-O-acetylscandoside, 6&beta, -hydroxy geniposide, daphylloside