NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (Suppl1): 60-62.

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Determination of Notoginsenoside R1 in Sanqi Xiaoshuan Tablets by HPLC

ZHOU Shu-qin1,2*,CHEN Qun-li1,2   

  1. 1 Shanghai Institute of Health Sciences,Shanghai 201318,China; 2 Affiliated Health School, College of Medicine,Shanghai Jiaotong University,Shanghai 201318, China
  • Online:2014-12-30 Published:2015-03-04

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Abstract: A HPLC method for the determination of notoginsenoside R1 in Sanqi Xiaoshuan Tablets was established.The separation was carried on a Phenomenex Luna C18 column(4.6×250 mm,5 μm)and acetonitrile-water was employed as the mobile phase in gradient elution mode.The flow rate was 1.0 mL/min with UV detected wavelength at 203 nm and the column temperature was set at 30 ℃.The linear range of notoginsenoside R1 was 0.02922~1.984 mg/mL(r=0.9999).The average recovery was 99.37% and RSD was 2.1%.The method is simple,accurate,specific and reproducible.It is suitable to the quality control of Sanqi Xiaoshuan Tablets.

Key words: Sanqi Xiaoshuan Tablets, Notoginsenoside R1, HPLC, preparation, sodium hydroxide solution

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