Abstract: In this study,the contents of peiminine in bulbs of Fritillaria thunbergii from 3 different areas:Zhangshui region at Ningbo city,Panan county at Jinhua city,Nantong county at Jiangsu provience and 3 different species:narrow leaves,broad leaves and multibulb cultivars were determined using high performance liquid chromatography (HPLC).Chromatographic separation was carried out on an Hypersil C₁₈ column (4.6 mm × 150 mm,5 μm) eluted with CH₃CN (A) and 100 mmol/L CH₃COONH₄ (pH 5.0) (B) at a flow rate of 1.0 mL/min.The detection wavelength was set at 375 nm.The sample volume was 10 μL.The HPLC specific chromatogram of F.thunbergii from different areas and species was primarily constructed and the content of peiminine was gained according to peak areas.The results showed that the contents of peiminine in F.thunbergii from Zhangshui region at Ningbo city,Panan county at Jinhua city,Nantong county at Jiangsu provience were 0.323%,0.281% and 0.194%,respectively.The contents of peiminine in narrow leaves,broad leaves,multi-bulb species of F.thunbergii were 0.317%,0.269% and 0.214%,respectively.HPLC metabolic profile developed by this experiment can be used to identify F.thunbergii from different areas and species.

Key words: Fritillaria thunbergii Miq, peiminine, HPLC analysis