NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (Suppl1): 87-92.doi: 10.16333/j.10016880.2016.S.022

• Article • Previous Articles     Next Articles

Pancreatin Hydrolysis Yak Casein and Priliminary Analysis of Physicochemical Property of Lactoalbumin Hydrolysate

JIN Dongwu1,2,LI Mingsheng1,3,ZHANG Jian2,LONG Shihe3,ZHANG Lei2, FENG Yuping1,2,LI Zhuo1,2,WEI Suocheng2,MA Zhongren1,2,3*   

  1. 1The Key Bioengineering and Technology Laboratory of Nationality Commission Northwest University 
    For Nationalities;2 Life Science and Engineering College of Northwest University for Nationalities;
    3Engineering Technology Research Center for Animal Cell Gansu,Lanzhou 730030,China
  • Online:2016-06-30 Published:2017-03-02

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Abstract: LH,which as compound bioactive peptide,has a wide range of applications in food nutrranceutical and biopharmaceuticals.The study using pancreatin hydrolysised YC to prepare YCLH.The physicochemical property of YCLH was investigated by SDSPAGE,SEM,FTIR,XRD and IEC.The results showed that the pancreatin add to YC with ratio of 1:5 to prepare YCLH under 40 ℃,pH 7.5 for 4 h.Consistent with HLH,there were 19 kinds of amino acids,the contents of total amino acids were 46.202±2.263% and 24.04±0.768%,the difference was extremely significant (P<0.01).There were no macromolecular protein,belong to matrix morphology,secondary structure were mainly existed in αhelix and βturns,contain protein (amid I),carboxyl,cellulose,nucleic acids,lipids and polysaccharide.In conclusion,this method is simple,time saving,feasibility to be industrialized,the physicochemical property of YCLH and HLH is extremely similar.The study can be served as a scientific basis for the further exploitation and utilization of the YC and the Localization of the LH.

Key words: pancreatin, yak casein, physicochemical property, lactoalbumin hydrolysate

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