NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2016, Vol. 28 ›› Issue (Suppl1): 84-86.doi: 10.16333/j.10016880.2016.S.021

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Determination of Cordycepin in Cultured Cordyceps Militaris by HPLC

DING Jie* LIU Jiun   

  1. Sichuan University of Science & Engineering(ZiGong 643000,Sichuan)
  • Online:2016-06-30 Published:2017-03-02

Abstract: This article uses the ultrasonic extraction of Cordycepin in cultured Cordyceps militaris,and adopting the high performance liquid chromatography (HPLC) method for the determination of cordycepin.When chromatographic column was ZORBAXExtendC18,water and methanol (95:5) as mobile phase,flow rate of 1.00 mL/min,detection wavelength of 260 nm,sample quantity 10 μL as a condition of chromatography,cordycepin in 10~1000 μg/mL has good linear relationship (S=3.3×104C-41890,R2=0.9999),the recovery rate:103.37 %,and RSD:2.26 %.

Key words: Cordyceps militaris fruiting body, Cordycepin, High performance liquid chromatography

CLC Number: