Abstract: To establish an efficient high performance liquid chromatography(HPLC)method for the simultaneous determination of rutin,isoquercitrin and quercetin in the leaves of Juniperus sabina L.Samples were analyzed on a Phenomenex GeminiNX C18∶〖KG-*2〗 column（250×4.6 mm,5 μm）eluted with acetonitrile(A)and 0.2% phosphoric acid solution(B)as the mobile phase in a gradient model;the column temperature was 30 ℃,the flow rate was 2.0mL/min,the detective wavelength was 360 nm.Rutin,isoquercitrin and quercetin in the leaves of Juniperus sabina L.were ideally separated in 25 min,and their linear response ranges for them were 10.4~62.4 μg/mL(r=0.9999),3.0~18.0 μg/mL(r=0.9998)and 11.0~66.0 μg/mL(r=0.9999),respectively.The average recovery rate was 98.64%,100.80% and 98.81% respectively.This method is simple,practicable and can be used as the quality control method of Juniperus sabina L.This method has been successfully applied to the analysis in different gathering periods of Juniperus Sabina L.The result showed that content of three components in Juniperus sabina were highest in the end of July.

Key words: HPLC, Juniperus Sabina L, rutin, isoquercitrin, quercetin