Abstract: The extraction conditions for ginkgolide from Ginkgo biloba leaves were optimized by response surface methodology.Extraction rate of Ginkgo Lactone as evaluation index,according to experiment of single factors,choose the levels.According to the BoxBehnken design experiment,the ExpertDesign software was used to analyze the data and get the best extraction conditions.Result showed that the optimal extraction conditions:extraction 3 times,time 2 hours,ethanol 44%,solidliquid ratio 0.04 g/mL,under these conditions,lactones of Ginkgo biloba extraction rate was 0.46%.Optimization of extraction conditions of Ginkgo biloba by response surface method,provide the guidance to actual production,reduce production costs and energy waste,the extraction efficiency is improved.

Key words: ginkgolides, response surface, extraction rate, Ginkgo biloba leaf