Abstract: The extract technology of the total flavonoids from Gnaphalium hypoleucum D.C.was optimized by orthogonal experiment with the yield of total flavonoids from G.hypoleucum as index,and the ethanol concentration,extracting time,the liquidsolid ratio as factors.The optimal extraction technology was as follows:70% ethanol,reflux extraction 2 times,each for 1 hour with liquidsolid ratio of 1:20.The content of total flavonoids was 10.74%.The enrichment and purification technology of total flavonoids with macroporous resin.Static adsorption and dynamic adsorption were used to optimize the adsorption and elution process by macroporous resin．X5 resin could effectively enrich the total flavonoids,the optimal conditions were:liquid concentration of 2.182.91 mg/mL,absorption velocity 1 mL/min,resin adsorption quantity 26.19 mg/g,eluting with 30% ethanol for 25BV,elution speed 3 mL/min.Under this condition,the content of total flavonoids increased to 42.41%,and the desorption rate was 84.05%.

Key words: Gnaphalium hypoleucum D.C., total flavonoids, extraction and purification technology, macroporous resin