NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2014, Vol. 26 ›› Issue (5): 656-661.

• Article • Previous Articles     Next Articles

Optimization of Ultrasonic-assisted Extraction of Phospholipid Fatty Acids from Forest Soil by Response Surface Analysis

LI Fan1, LI Na2*, CHEN Jian-zhong2, JIANG Xiao-qiang2, CHEN Yi2, LI Ping1   

  1. 1 Southwest Jiaotong University,Chengdu 610031,China;2 Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China
  • Online:2014-05-31 Published:2014-11-17

Abstract: The objective of this study was to optimize ultrasonic-assisted extraction of phospholipid fatty acids (PLFAs) from forest soil using response surface methodology (RSM). Based on single factor experiments,extraction temperature,time and amount of extraction solvent were selected for Box-Behnken central composite designing. RSM was employed to study the effect of these factors on the yield of PLFAs.The results indicated that the optimal extraction conditions were 20 ℃ as extraction temperature,21 mL as amount of extraction solvent,with 30 min of extraction.Under these conditions,the maximal extraction yield reached 45.57 nmol/g (n=3).The response surface methodology used for the optimization of ultrasonic-assisted extraction conditions of PLFAs from forest soil was simple and reliable.

Key words: PLFAs, ultrasonic extraction, response surface methodology

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