Abstract: One active compound was extracted from Celastrus aculeatus Merr.,and then purified by HPLC,finally it was identified as nimbidiol by 1H NMR and  13C NMR.In order to study its potential anti-angiogenesis effect,zebrafish model and rat arterial ring experiment was used in this research.Nimbidiol was given to zebrafish embryos and rat arterial rings with final concentrations of 12.5 μmol/L,25 μmol/L and 50 μmol/L.After co-incubation,the development situation of intersegmental blood vessels (ISV) and new blood vessels formation around artery rings was observed under a microscope,then photographed for statistic analyzing.Study results showed that nimbidiol suppressed ISV development,in each nimbidiol group,average means of ISV decreased with statistically difference compared with negative control group (F=24.773, P<0.05).At the same time,nimbidiol also can inhibit endothelial cell proliferation and new blood vessels formation around artery rings.Taking all the results in conclusion that nimbidiol had dosage depended anti-angiogenesis effect.

Key words: Celastrus aculeatus Merr., nimbidiol, zebrafish, rats aortic rings, angiogenesis