NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2015, Vol. 27 ›› Issue (12): 2095-2100. doi: 10.16333/j.1001-6880.2015.12.019

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Hydrolysis of Fructus Sophorae Isoflavones by Hydrochloric Acid Catalysis

ZHANG Xiao-song,ZHANG Bo-ya, JIN Hua*, ZHANG Yong-zhong   

  1. Application Chemistry Department of Northeast Agriculture University,Harbin 150030,China
  • Online:2015-12-29 Published:2016-01-06

Abstract: Fructus Sophorae isoflavones were hydrolyzed to genistein in hydrochloric acid-methanol solution.The hydrolysis rate of Fructus Sophorae isoflavones was detected by high performance liquid chromatography.The effects of hydrolysis time,hydrolysis temperature and hydrochloric acid concentration on hydrolysis rate of Fructus Sophorae isoflavones were investigated.The optimal hydrolysis conditions were determined as follows:hydrolysis time 3.8 h,hydrochloric acid concentration 2.59 mol/L,hydrolysis temperature 78.5 ℃.Under these conditions,the hydrolysis rate of total isoflavones reached 93.38%.

Key words: Fructus Sophorae isoflavones, acid hydrolysis, high performance liquid chromatography

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