原花青素B2通过PI3K/PPARα/Plin5介导的脂滴脂解在体内外抗脂质积聚的研究

doi:10.16333/j.1001-6880.2026.3.014

天然产物研究与开发 ›› 2026, Vol. 38 ›› Issue (3): 581-589.doi: 10.16333/j.1001-6880.2026.3.014 cstr: 32307.14.1001-6880.2026.3.014

原花青素B2通过PI3K/PPARα/Plin5介导的脂滴脂解在体内外抗脂质积聚的研究

郭伊玲，李彦志，曹莹琪，李芸霞*

成都中医药大学药学院西南特色中药资源国家重点实验室，成都 611137

出版日期:2026-03-27 发布日期:2026-03-26
基金资助:
国家自然科学基金（81891012，U19A2010，81630101）；国家中医药管理局创新团队和人才培养计划（ZYYCXTD-D-202209）

Procyanidin B2 alleviates lipid accumulation in vivo and in vitro via PI3K/PPARα/Plin5-mediated lipolysis of lipid droplets

GUO Yi-ling,LI Yan-zhi,CAO Ying-qi,LI Yun-xia*

State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China

Online:2026-03-27 Published:2026-03-26

摘要/Abstract

摘要：

研究原花青素B2（procyanidin B2，PB2）在体内外抗脂滴积聚的潜在机制。喂养高脂饮食获得脂质代谢紊乱小鼠模型并用游离脂肪酸诱导脂滴积聚细胞模型，以不同浓度的PB2给药干预。中、高剂量PB2（75、100 mg/kg）降低了高脂饮食诱导小鼠的体重和肝脏指数，并改善了血清与肝脏脂质谱（P < 0.01或P < 0.001）。体外结果显示，PB2减少了细胞内甘油三酯含量（P < 0.001）。油红O染色结果显示PB2在体内外缓解了脂滴积聚，减少了其数量与密度。Western blot分析显示，中、高剂量PB2下调了围脂素5（perilipin 5，Plin5）的表达（P < 0.01或P < 0.001）；此外，Plin5的上游蛋白过氧化物酶体增殖激活受体α（peroxisome proliferator-activated receptor α，PPARα）和磷脂酰肌3-激酶（phosphatidylinositol 3-kinase，PI3K）磷酸化的表达水平均被抑制。上述结果表明，通过PI3K/PPARα/Plin5通路介导的脂滴脂解可能是PB2减轻脂滴过度积聚的重要机制之一。

关键词: 原花青素B2, 脂肪变性, 脂滴, 脂解, 围脂素5

Abstract:

This study aims to investigate the potential mechanism of procyanidin B2 (PB2) against lipid droplets accumulation in vitro and in vivo. Mice were induced to develop lipid metabolism disorders by feeding a high-fat diet (HFD) and induced intracellular lipid droplets deposition with free fatty acids. They were intervened by the administration of PB2 at different concentrations. Medium and high doses of PB2 (75 and 100 mg/kg) significantly reduced body weight and liver index and improved serum and liver lipid profile in HFD-induced mice (P < 0.01 or P < 0.001). In vitro results showed that PB2 decreased intracellular triglyceride content (P < 0.001). Oil red O staining showed that PB2 alleviated lipid droplets accumulation and reduced their number and density in vitro and in vivo. Western blot analysis showed that medium and high doses of PB2 down-regulated the expression of perilipin 5 (Plin5) (P < 0.01 or P < 0.001). In addition, the expression levels of the upstream proteins of Plin5, peroxisome proliferator-activated receptor α (PPARα) and phosphatidylinositol 3-kinase (PI3K) phosphorylation, were inhibited. The above results suggest that lipolysis of lipid droplets mediated through the PI3K/PPARα/Plin5 pathway may be one of the important mechanisms by which PB2 attenuates the excessive accumulation of lipid droplets.

Key words: procyanidin B2, steatosis, lipid droplets, lipolysis, perilipin 5

中图分类号: R285.5

郭伊玲, 李彦志, 曹莹琪, 李芸霞. 原花青素B2通过PI3K/PPARα/Plin5介导的脂滴脂解在体内外抗脂质积聚的研究[J]. 天然产物研究与开发, 2026, 38(3): 581-589.

GUO Yi-ling, LI Yan-zhi, CAO Ying-qi, LI Yun-xia. Procyanidin B2 alleviates lipid accumulation in vivo and in vitro via PI3K/PPARα/Plin5-mediated lipolysis of lipid droplets[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(3): 581-589.

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原花青素B2通过PI3K/PPARα/Plin5介导的脂滴脂解在体内外抗脂质积聚的研究

Procyanidin B2 alleviates lipid accumulation in vivo and in vitro via PI3K/PPARα/Plin5-mediated lipolysis of lipid droplets

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