三七根总皂苷、总黄酮、总多糖抗氧化活性对比研究

doi:10.16333/j.1001-6880.2026.9.005

天然产物研究与开发 ›› 2026, Vol. 38 ›› Issue (9): 1915-1924.doi: 10.16333/j.1001-6880.2026.9.005 cstr: 32307.14.1001-6880.2026.9.005

三七根总皂苷、总黄酮、总多糖抗氧化活性对比研究

黄加文 1,2,3,何志明 1,2,3,詹易 1,2,3,吴德松 1,2,3,查雨锋 1,2,3∗

1云南白药集团股份有限公司,昆明650500; 2云南省药物研究所;3 云南省中药和民族药新药创制企业重点实验室,昆明650111

出版日期:2026-09-24 发布日期:2026-09-22
基金资助:
云南特色植物筛选与研发服务CXO 平台建设(2022YKZY001);云南省基础研究专项面上项目(202401AT070155)

Comparison on antioxidant activities of total saponins,total flavonoids, and total polysaccharides from Panax notoginseng roots

HUANG Jia-wen1,2,3,HE Zhi-ming1,2,3,ZHAN Yi1,2,3,WU De-song1,2,3,ZHA Yu-feng1,2,3 ∗

1Yunnan Baiyao Group Co. ,Ltd. ,Kunming 650500,China;2Yunnan Institute of Materia Medica; 3Yunnan Province Company Key Laboratory for TCM and Ethnic Drug of New Drug Creation,Kunming 650111,China

Online:2026-09-24 Published:2026-09-22

摘要/Abstract

摘要：

探究三七根总皂苷、总黄酮、总多糖体外抗氧化活性的差异。提取三七根中的上述3 种成分,采用DPPH、 ABTS 自由基清除实验比较其自由基清除能力;采用H₂O₂诱导人皮肤成纤维细胞(human foreskin fibroblasts,HFF)氧化损伤模型,通过检测丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、还原型谷胱甘肽(reduced glutathione,GSH)、活性氧(reactive oxygen species,ROS)等细胞氧化应激指标及I 型胶原蛋白(type I collagen,COL-I)、COL-Ⅲ、基质金属蛋白酶1(matrix metalloproteinase 1,MMP-1)、MMP-3 蛋白表达,比较其改善氧化应激的能力;采用中波紫外线(ultraviolet B,UVB)诱导3D 皮肤损伤模型,通过HE 染色观察及检测紧密连接蛋白-1(claudin-1,CLDN-1)、闭锁小带蛋白-1(zonula occludens-1,ZO-1)蛋白表达,比较其屏障修复能力,综合评估其抗氧化活性。结果表明:在自由基清除活性方面,总皂苷表现微弱,总黄酮的清除能力优于总多糖;在抗氧化应激方面,各提取成分均能不同程度降低细胞中MDA 含量、提升SOD 活性与GSH 含量、抑制ROS 生成、促进COL-Ⅰ和COL-Ⅲ的表达、抑制MMP-1 和MMP-3 的表达(P < 0. 05,P < 0. 01,P < 0. 001),其中总皂苷与总黄酮的抗氧化应激能力相当,总多糖较弱;在屏障修复方面,各提取成分均能修复UVB 所致皮肤屏障损伤,显著上调CLDN-1 与ZO-1 的表达(P < 0. 01,P < 0. 001),总皂苷的屏障修复能力最优,其次为总黄酮,总多糖最弱。本研究系统比较了三七根总皂苷、总黄酮、总多糖的体外抗氧化活性差异,证实了三七根不同成分在抗氧化方面各有侧重且相互补充,为三七资源的高值化利用提供了理论依据。

关键词:

"> 三七根, 自由基清除, 氧化应激, 屏障修复, 3D 皮肤模型

Abstract:

To investigate the differences in antioxidant activities of total saponins,total flavonoids,and total polysaccharides from Panax notoginseng roots in vitro,these three components were extracted and their free radical scavenging abilities were compared using DPPH and ABTS assays. The capacity to alleviate oxidative stress was evaluated using an H₂O₂-induced human foreskin fibroblasts ( HFF) cell model by measuring cellular oxidative stress markers including malondialdehyde (MDA),superoxide dismutase (SOD),reduced glutathione (GSH),and reactive oxygen species (ROS),as well as protein expression levels of type I collagen (COL-Ⅰ),COL-Ⅲ,matrix metalloproteinase 1 (MMP-1) and MMP-3. Additionally,a 3D skin damage model induced by UVB radiation was used to assess barrier repair ability through HE staining and detection of claudin-1 (CLDN-1) and zonula occludens-1 (ZO-1) protein expression. The antioxidant activity of each component was comprehensively evaluated. Results showed that in terms of free radical scavenging ability,total saponins exhibited weak activity,while total flavonoids demonstrated superior scavenging capacity compared to total polysaccharides. Regarding antioxidant stress,all extracts significantly reduced intracellular MDA levels,enhanced SOD activity and GSH content,suppressed ROS generation,promoted COL-Ⅰand COL-Ⅲexpression,and inhibited MMP-1 and MMP-3 expression (P < 0. 05,P < 0. 01,P < 0. 001). Among them,total saponins and total flavonoids showed comparable antioxidant effects,whereas total polysaccharides were relatively weaker. In barrier repair,all extracts effectively repaired UVB-induced skin barrier damage and significantly upregulated CLDN-1 and ZO-1 expression (P < 0. 01,P < 0. 001),with total saponins showing the strongest barrier repair effect,followed by total flavonoids,and total polysaccharides being the weakest. This study systematically compared the in vitro antioxidant activities of total saponins,total flavonoids,and total polysaccharides from P. notoginseng roots,confirming that different components exhibit distinct yet complementary antioxidant functions,providing theoretical support for the highvalue utilization of P. notoginseng resources.

Key words:

"> Panax notoginseng roots, free radical scavenging, oxidative stress, skin barrier repair, 3D skin model

中图分类号:

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黄加文, 何志明, 詹易, 吴德松, 查雨锋. 三七根总皂苷、总黄酮、总多糖抗氧化活性对比研究[J]. 天然产物研究与开发, 2026, 38(9): 1915-1924.

HUANG Jia-wen, HE Zhi-ming, ZHAN Yi, WU De-song, ZHA Yu-feng. Comparison on antioxidant activities of total saponins,total flavonoids, and total polysaccharides from Panax notoginseng roots[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(9): 1915-1924.

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链接本文: http://www.trcw.ac.cn/CN/10.16333/j.1001-6880.2026.9.005

http://www.trcw.ac.cn/CN/Y2026/V38/I9/1915

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三七根总皂苷、总黄酮、总多糖抗氧化活性对比研究

Comparison on antioxidant activities of total saponins,total flavonoids, and total polysaccharides from Panax notoginseng roots

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